Revolutionizing Histological Imaging with Advanced Microscopy Techniques

The text is a summary of a thesis titled "Rapid and non-destructive histological imaging of large clinical specimens with translational and advanced optical microscopy" by Yan Zhang. The thesis proposes four imaging techniques, including computational high-throughput autofluorescence microscopy by pattern illumination (CHAMP), microscopy with ultraviolet single-plane illumination (MUSI), microtomy-assisted autofluorescence tomography (MATE), and wide-field fluorescence-based histological imaging with High-and-Low-frequency microscopy (HiLo). These techniques enable rapid, label-free, and non-destructive imaging of tissues, with potential to revolutionize the current practice of surgical pathology.